Getting Started with MacVector: An overview of primer design workflows in MacVector.Melissa Caimano on HOW DO I video guides to common molecular biology workflows.admin on HOW DO I video guides to common molecular biology workflows.mariam abdelmalak on Major release details – Summary.Brian on Designing primers and documenting In-Fusion Cloning with MacVector.Chris on Designing primers and documenting In-Fusion Cloning with MacVector.MacVectorTip: correctly flagging PacBio and Oxford Nanopore datasets for assembly by Flye.MacVectorTip: visualizing shared domains in a protein alignment.
Macvector reverse alignment software#
MacVectorTip: displaying CRISPR PAM Sites on a sequence Ahead and reverse primer sequences for mouse specific genes have been created utilizing MacVector software (MacVector Inc.MacVectorTip: Sign up for an NCBI API key to speed up BLAST results.MacVectorTip: Designing Primers for Gibson Assembly.When you switch to the Picture tab, you will see colored outlines around the shared domains. In the Editor tab a new line will appear above each sequence displaying the extent and color of visible features. In the EDITOR tab using the toolbar button turn on the feature display MODE to SHOW FEATURES.Now run the alignment by clicking ALIGN.Click OPTIONS (bottom left hand corner) and choose OPEN MULTIPLE SEQUENCE FILE – AS MULTIPLE ALIGNMENT.Use FILE | OPEN and select multiple protein sequences.Ensure your protein sequences are annotated and that the domains of interest are visible (you can use DATABASE | INTERPROSCAN to quickly scan and annotate domains to your proteins).Note that alignments created using versions of MacVector before 17.5 will not have this information and will need to be recreated. You can also create new domains and dynamically show/hide features in alignments. The colors of features from the individual sequence documents are used to outline the domains in the alignment. MacVector’s new multiple alignment file format retains the features/annotations from the sequences that are used to create the alignment. MacVector has a domain-outlining facility for multiple sequence alignments, letting you easily visualize the relationships between features in aligned protein sequences.
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